Prof. Eske Willerslev and a colleague sample sediments for environmental DNA in Greenland. Credit: Handful of Films
Prof. Eske Willerslev and a colleague sample sediments for environmental DNA in Greenland. Credit: Handful of Films

World’s oldest DNA reveals a two million year old ecosystem

Embargoed until: Publicly released:
Peer-reviewed: This work was reviewed and scrutinised by relevant independent experts.

The world's oldest DNA has been found in Ice Age sediment in northern Greenland, revealing the plants and animals that were part of this ancient ecosystem. The two-million-year-old DNA is a million years older than the previous record for DNA sampled from a Siberian mammoth bone. The analysis by researchers, including an Australian, showed that some of the DNA fragments were from the predecessors of present-day species, but some came from species that were impossible to place in the DNA libraries of animals, plants and microorganisms still living in the 21st century. The scientists discovered evidence of reindeer, hares, lemmings, birch and poplar trees, and even found evidence of Mastodon, an Ice Age mammal, which was previously unknown to be in Greenland. 

Journal/conference: Nature

Link to research (DOI): 10.1038/s41586-022-05453-y

Organisation/s: Curtin University, University of Cambridge, UK

Funder: We acknowledge support from the Carlsberg Foundation for logistics to carry out two expeditions to Kap København in 2006 and 2012 (S. Funder, principal investigator for Carlsberg foundation grant to LongTerm and Kap København—the age). The fieldwork in 2016 was supported by a grant to N.K.L. from the Villum Foundation. We highly appreciate the collaborative support by Illumina Inc. that was crucial for the success of the project. E.W. and K.H.K. thank the Danish National Research Foundation (DNRF) and the Lundbeck Foundation (R302-2018-2155) for providing long-term funds to develop the necessary DNA technology that eventually made it possible to retrieve environmental DNA from these ancient deposits in the Kap København Formation. E.W. also acknowledges the Wellcome Trust (UNS69906), the Carlsberg Foundation (CF18-0024), Novo Foundation (NNF18SA0035006), Leverhume (RPG-2016-235) and GRF EXC CRS Chair - Cluster of Excellence (44113220) for their support. M.W.P. acknowledges support from the Carlsberg Foundation (CF17-0275). K.K.S. and S.J. acknowledge support from VILLUM FONDEN (00025352). I.G.A. and E.C. have received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 819192). B.D.S. acknowledges support from the Wellcome Trust programme in Mathematical Genomics and Medicine (WT220023). J.Å.K. was supported by the Carlsberg Foundation (CF20-0238). C.B. acknowledges ERC Advanced Award Diatomic (grant agreement no. 835067). J.C.G. was supported by Natural Science and Engineering Research Council of Canada–Discovery Grant 06785 and Canada Foundation for Innovation grant 21305. M.J.C. acknowledges support from the Danish National Research Foundation DNRF128.

Media release

From: Springer Nature

Make-up of a two-million-year-old ecosystem reconstructed from ancient DNA *IMAGES* *PRESS BRIEFING* 

An analysis of the oldest ancient environmental DNA recovered to date has been used to build a picture of an ecosystem in North Greenland, including the animal and plant species present, approximately two million years ago. The findings, published in Nature, allow us to explore and understand an ancient ecosystem to an extent not previously possible and provide insights into an ecosystem that has no modern equivalent.

The Kap København Formation, located in Peary Land, North Greenland, is a polar desert. Previous research suggests that the region had a much warmer climate around 2–3 million years ago with temperatures 11–19 °C warmer than today. However, the biological communities inhabiting the Arctic during this time remain poorly understood because vertebrate fossils are rare.

Eske Willerslev and colleagues extracted and sequenced DNA from 41 organic-rich sediment samples taken from 5 different sites within the Kap København Formation. From this DNA, the authors were able to build a picture of the ancient ecosystem: an open boreal forest with a mixed vegetation of poplar, birch and thuja trees, as well as a variety of Arctic and boreal shrubs and herbs. The DNA record also confirms the presence of hares, and mitochondrial DNA from the site points to the presence of other animals including mastodons, reindeer, rodents and geese. Ancient DNA was also recovered from marine organisms and suggests the presence of a population of the Atlantic horseshoe crab (Limulus polyphemus). The authors propose that this could mean that there were warmer surface water conditions in the Early Pleistocene at Kap København, which is consistent with previous estimates.

The authors conclude that their findings demonstrate the potential to use ancient environmental DNA to track the evolution of biological communities two million years ago.

B-roll and further images available on request from press@nature.com

**Please note that an online press briefing for the papers below will take place UNDER STRICT EMBARGO on Tuesday 06 December at 3pm London time (GMT) / 10am US Eastern Time**

Authors Eske Willerslev, Kurt Kjær, Nicolaj Larsen, Karina Sand, Mikkel Pedersen, and Bianca DeSanctis will discuss the research. This will be followed by a Q&A session.

To attend this briefing you will need to pre-register by following the link here. Once you are registered, you will receive an email containing the details for the briefing. You will also be provided with the option to save the details of the briefing to your calendar.

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  • A sample collected by Prof. Eske Willerslev in Greenland.
    A sample collected by Prof. Eske Willerslev in Greenland.

    A sample collected by Prof. Eske Willerslev in Greenland.

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  • A researcher extracts samples from a sediment core for DNA sequencing
    A researcher extracts samples from a sediment core for DNA sequencing

    A researcher extracts samples from a sediment core for DNA sequencing

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